Molecular+Biology+4


 * Assessment Items from the Molecular Biology 4 Small Group**


 * Learning objective (very general) - gene expression**


 * Learning outcomes:**


 * 1) Predict based on a model of protein-protein and protein-DNA interactions in gene expression, how alterations in either DNA or protein components impact expression of the target (eukaryotic) gene**


 * 2) Given a malfunction a malfunction in gene regulation (no expression), what mutations can account for lack of expression?**


 * 3) Explain how cell signals affect regulation of gene expression**


 * 4) Describe how gene regulation results in cells with different structures and functions**


 * 5) Develop a model for how a unicellular organism can respond to changes in the environment**


 * Summative assessments**


 * Illustration of eukaryotic promoter with general TFs with promoter, enhancer**


 * Note: for assessment, remove or alter labels as needed: for example, upstream enhancer could be labeled Region A**


 * Shown is a model of the regulatory system for expression of one gene. Answer the following questions about gene expression.**

3. What other binding events must occur prior to enzyme binding? 4. If this is a gene whose expression is regulated, what is the function of the element(s) labeled region A? 5. What can bind to these elements?
 * 1. CIrcle the region necessary for expression of all genes, even those that are not regulated.**
 * 2. ** What enzyme binds to that region?

LO1 Formative assessment

A patient has an inherited disorder where the gene is not functional. However, sequencing reveals that there is no alteration in the coding sequence of the gene. Mutations in what other locations could cause the loss of this gene function?


 * LO2) Explain how cell signals affect regulation of gene expression. **

LO2 Summative questions:

Gene Y has a testosterone-response element in its promoter. Gene Y is expressed in muscle cells, but not in liver cells.
 * 1) Explain why liver cells do not express gene Y.
 * 2) A patient fails to express gene Y in muscle cells. Propose at least two hypotheses to explain the lack of expression of gene Y in this patient.

LO2 Formative assessment  Construct an interactive case study similar to summative assessment, in a different context. Ask how to test each hypothesis.