Molecular+Biology+1

Goal: Understand gene expression.
 * Assessment Items from the Molecular Biology 1 Small Group**

Learning outcome: Given a particular DNA sequence, students will be able to predict the mRNA and amino acid sequences.

Summative assessment:

1. Below is a sequence of DNA that encodes a polypeptide. The positions of the start and stop codons are indicated. Which strand is transcribed into mRNA and in which direction?

-TCA--CAT-- -AGT--GTA--

a. Top strand; left to right b. Top strand; right to left c. Bottom strand; left to right d. Bottom strand; right to left

2. Circle all of the mRNA sequences below that encode the following amino acid sequence. The mRNA sequences are given 5’-3’ from left to right.

Met-Ser-Arg-Leu

a. AUGAGUCGUCUU b. AUGAGUAAUCUG c. AUGAGAAGCCUA d. AUGAGCCGACUG

Formative assessment (Practice going forward and backward through central dogma) The table below represents a short sequence of DNA that encodes a very short polypeptide. Use the information provided to complete the table. Hint: First find the start codon.
 * DNA || 5’ ||  || ATG ||   ||   ||   ||   || CCG || 3’ ||
 * || 3’ || ATG ||  ||   ||   ||   || ATC ||   || 5’ ||
 * mRNA ||  ||   ||   || GCA || AGU ||   ||   ||   ||   ||
 * Amino acid ||  ||   ||   ||   ||   || Trp ||   ||   ||   ||